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MOLECULAR BIOLOGY: WORKING WITH PROTEINS

PROTEIN EXPRESSION: LABELING

1. In Vivo Labeling of Plant Proteins and Immunoprecipitation
2. 35S-Methionine Labeling of Yeast Cells
3. Isolation of 32P-labeled Protein for Phosphoamino Acid and Phosphopeptide Analyses.
4. Labeling Muscle Actin with Carboxyfluorescein Succinimidyl Ester
5. Labeling Tubulin with Fluorescent Dye and Quantifying Labeling Stoichiometry
6. Measurement of Incorporation of 35S-Cysteine into Cellular Protein
7. NaOH-Peroxide Method to Determine the Incorporation of Radiolabeled Amino Acids into Protein
8. Overexpression of Recombinant Proteins in COS-7 Cells
9. Preparation of Radiolabeled 14C-Acetylated Collagen
10. Preparation of Rhodamine-Labeled Actin
11. Steady State Labeling with [35S] Trans Label in Drosophila L2 Cells
12. TCA Precipitation to Determine the Incorporation of Radiolabeled Amino Acids into Protein





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